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Open Access Research

Diversity of Bacillus-like organisms isolated from deep-sea hypersaline anoxic sediments

Andrea M Sass12, Boyd A McKew1, Henrik Sass3, Jörg Fichtel4, Kenneth N Timmis156 and Terry J McGenity1*

Author Affiliations

1 Department of Biological Sciences, University of Essex, Wivenhoe Park, Colchester CO4 3SQ, UK

2 School of Biosciences, Cardiff University, Cardiff CF10 3YE, UK

3 School of Earth Ocean & Planetary Science, Cardiff University, Cardiff CF10 3YE, UK

4 Institute for Chemistry and Biology of the Marine Environment (ICBM), University of Oldenburg, D-26111 Oldenburg, Germany

5 Helmholtz Center for Infection Research, Inhoffenstrasse 7, D-38124 Braunschweig, Germany

6 Institute for Microbiology, Technical University Braunschweig, Germany

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Saline Systems 2008, 4:8  doi:10.1186/1746-1448-4-8

Published: 9 June 2008

Abstract

Background

The deep-sea, hypersaline anoxic brine lakes in the Mediterranean are among the most extreme environments on earth, and in one of them, the MgCl2-rich Discovery basin, the presence of active microbes is equivocal. However, thriving microbial communities have been detected especially in the chemocline between deep seawater and three NaCl-rich brine lakes, l'Atalante, Bannock and Urania. By contrast, the microbiota of these brine-lake sediments remains largely unexplored.

Results

Eighty nine isolates were obtained from the sediments of four deep-sea, hypersaline anoxic brine lakes in the Eastern Mediterranean Sea: l'Atalante, Bannock, Discovery and Urania basins. This culture collection was dominated by representatives of the genus Bacillus and close relatives (90% of all isolates) that were investigated further. Physiological characterization of representative strains revealed large versatility with respect to enzyme activities or substrate utilization. Two third of the isolates did not grow at in-situ salinities and were presumably present as endospores. This is supported by high numbers of endospores in Bannock, Discovery and Urania basins ranging from 3.8 × 105 to 1.2 × 106 g-1 dw sediment. However, the remaining isolates were highly halotolerant growing at salinities of up to 30% NaCl. Some of the novel isolates affiliating with the genus Pontibacillus grew well under anoxic conditions in sulfidic medium by fermentation or anaerobic respiration using dimethylsulfoxide or trimethylamine N-oxide as electron acceptor.

Conclusion

Some of the halophilic, facultatively anaerobic relatives of Bacillus appear well adapted to life in this hostile environment and suggest the presence of actively growing microbial communities in the NaCl-rich, deep-sea brine-lake sediments.