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Open Access Research

Hematodinium sp. and its bacteria-like endosymbiont in European brown shrimp (Crangon crangon)

Grant D Stentiford1*, Kelly S Bateman1, Hamish J Small2, Michelle Pond1 and Anette Ungfors3

Author Affiliations

1 European Union Reference Laboratory for Crustacean Diseases, Centre for Environment, Fisheries and Aquaculture Science (Cefas), Barrack Road, Weymouth, Dorset, DT4 8UB, United Kingdom

2 Virginia Institute of Marine Science, College of William and Mary, P.O. Box 1346, Gloucester Point, VA, 23062, USA

3 Department of Biology and Environmental Sciences, Tjärnö, University of Gothenburg, Strömstad, SE, 452 96, Sweden

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Aquatic Biosystems 2012, 8:24  doi:10.1186/2046-9063-8-24

Published: 7 September 2012

Abstract

Background

Parasitic dinoflagellates of the genus Hematodinium are significant pathogens affecting the global decapod crustacean fishery. Despite this, considerable knowledge gaps exist regarding the life history of the pathogen in vivo, and the role of free living life stages in transmission to naïve hosts.

Results

In this study, we describe a novel disease in European brown shrimp (Crangon crangon) caused by infection with a parasitic dinoflagellate of the genus Hematodinium. This is the second example host within the Infraorder Caridea (shrimp) and significantly, the first description within the superfamily Crangonoidea. Based upon analysis of the rRNA gene (SSU) and spacers (ITS1), the parasite in C. crangon is the same as that previously described infecting Nephrops norvegicus and Cancer pagurus from European seas, and to the parasite infecting several other commercially important crab species in the Northern Hemisphere. The parasite is however distinct from the type species, H. perezi, found infecting type hosts (Carcinus maenas and Liocarcinus depurator) from nearby sites within Europe. Despite these similarities, the current study has also described for the first time, a bacteria-like endosymbiont within dinospore stages of the parasite infecting shrimp. The endosymbionts were either contained individually within electron lucent vacuoles within the parasite cell cytoplasm, or remained in direct contact with the parasite cytoplasm or in some cases, the nucleoplasm. In all of these cases, no apparent detrimental effects of colonization were observed within the parasite cell.

Conclusions

The presence of bacteria-like endosymbionts within dinospore life stages presumes that the relationship between the dinoflagellate and the bacteria is extended beyond the period of liberation of spores from the infected host shrimp. In this context, a potential role of endosymbiosis in the survival of free-living stages of the parasite is possible. The finding offers a further intriguing insight into the life history of this enigmatic pathogen of marine crustacean hosts and highlights a potential for mixotrophy in the parasitic dinoflagellates contained within the genus Hematodinium.

Keywords:
ITS1; Phylogenetics; Dinoflagellate; Bacteria; Crustacean; Disease; Fishery